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By Oscar; Latner, A. L. Bodansky

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4. The absorbance signal is electronically differentiated to obtain its rate 34 D. W. MOSS FIG. 15. The Beckman System TR enzyme activity analyzer. ) of change with time, and the variation of the first differential with time is monitored by a second differentiating circuit (Fig. 9). , the reaction is following zeroorder kinetics. After the rapid fluctuations in absorbance due to reaction initiating and mixing have subsided, the second differential is monitored until a zero value lasting for 17 seconds is registered.

Mixing within the cuvette is by a magnetic stirrer. 1"Cat 37°C or, with external cooling, at 30°C or 25°C. Monitoring wavelengths of 405 k 1 nm or 340 1 nm (bandwidths < 5 nm) isolated by a diffraction grating are selected as appropriate by the push button which programs for each type of assay. The second, reference cuvette of the double-beam photometer normally contains air; however, a dual-probe sample pick up is provided so that a sample or reagent blank can be used to compensate for possible errors introduced into some assays by the use of serum initiation.

In these instruments the individual reaction mixtures enter measurement cuvettes by centrifugal action. The cuvettes pass through the light beam of the photometer once in every revolution of the reaction rotor. For a rotation speed of 600 rpm, 10 readings are taken from each cuvette every second. Computer circuits are needed to record and analyze these large inputs of data. The Beckman Instruments, Inc. System TR enzyme activity analyzer employs a different method of determining the slope and linearity of the progress curve.

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