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Download Atlas of Clinical Haematology by Dr. med., Prof. Herbert Begemann, Dr. med., Priv.-Doz. PDF

By Dr. med., Prof. Herbert Begemann, Dr. med., Priv.-Doz. Johann Rastetter (auth.)

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The nucleus of these cells is, similar to the myeloblasts, delicate and still shows several (up to 6) nucleoli. The cells are peroxidase positive. However the granules seen on panoptic staining and those of the peroxidase reaction are not identical. The size of the cell fluctuates between 20 and 25 Jlm. Thus these cells are the largest of both myeloid and erythroid series. Mitoses are absolutely more frequent than with myeloblasts, the mitotic index (mitotic index = number of mitoses of a certain type of cell in relation to 1000 of these cells) is smaller.

7. Gold chloride solution for 10 min. Wash with distilled water. 8. Potassium metabisulphite solution for 1 min. 9. Sodium thiosulphate solution for 1 min. Wash thoroughly with tap water. Dry. If smears are carefully handled mounting in Canada balsam is not necessary. Giemsa or Pappenheim stained smears may subsequently be impregnated by this method. The reticulin fibres appear dark brown to black. 2. Staining Methods for the Demonstration of Blood Parasites Staining of the "Thick Drop" Place one or two drops of blood onto a slide and spread by means of a small lancet to ca 15 mm diameter (not too thick since the "thick drop" might split off on drying or float off on staining).

If smears are carefully handled mounting in Canada balsam is not necessary. Giemsa or Pappenheim stained smears may subsequently be impregnated by this method. The reticulin fibres appear dark brown to black. 2. Staining Methods for the Demonstration of Blood Parasites Staining of the "Thick Drop" Place one or two drops of blood onto a slide and spread by means of a small lancet to ca 15 mm diameter (not too thick since the "thick drop" might split off on drying or float off on staining). Dry in air, stain only when perfectly dry.

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